Thermo Scientific Pierce Sulfo-NHS （N-hydroxysulfosuccinimide） and its uncharged analog NHS （N-hydroxysuccinimide） are chemical modification reagents for converting carboxyl groups to amine-reactive Sulfo-NHS esters for application in a variety of bioconjugation and crosslinking methods.

Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC （EDAC）. The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
 

Highlights:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
• Amine-reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule
• Sulfo-NHS derivatives are water-soluble, so crosslinking can be done in physiologic solutions
• High-purity, crystalline Sulfo-NHS can be used to create high-purity activated derivatives