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重組人白介素-2 (rHuIL-2)

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更新時(shí)間:2025-05-02 15:04:05瀏覽次數(shù):1110次

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CYT-209重組人白介素-2 (rHuIL-2) 10μg/50μg/1mg Prospec

 

 

Description :
 Recombinant Human IL-2 produced in E.Coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton.

Our rHuIL-2 has an Ser substitute for Cysteine at position 126. The rHuIL-2 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic & 0.89mg dibasic sodium phosphate buffer to a pH=7.5.

 
Solubility:
 It is recommended to reconstitute the lyophilized IL-2 in sterile 20mM acetic acid (AcOH) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized rHuIL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec's rHuIL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.0645 ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of HuIL-2.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 


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